The quality of a leader is best understood from the standard he sets for himself
Consultant Rheumatologist and Director, Research
M. D. (Internal Medicine), FICP (India)
The Biochemistry Laboratory is well equipped and is in accordance keeping with the progressive outlook of the hospital. The laboratory has sophisticated state-of-the-art analyzers, Beckman Lx20 and the Synchron Cx7, which have, CAP piercing continuous loading and reporting facilities besides having the primary tube sampling facilities and Bar Code identification systems.
The entire process is integrated through Remisol and LIS. The Lx-20 is capable of handling 1200 tests per hour. The instruments are capable of processing urgent samples (STAT) and can report the value of 9 parameters within less than a minute, thus making critical information available quickly for emergency samples.
The section has the ELISE, Radiometer, Blood Gas Analyzer, Glucose II Analyser, ES-300- the automated walk away immunoassay system, TDX Analyser, LKB and Sebia Electrophoresis Unit, and Beckman Protein Array to determine small quantities of different proteins by rate nephelometry, System 700 & the Toxi Lab System.
The test menu covers over 180 different investigations ranging from the most elementary tests like glucose and electrolytes done by the state-of-the-art techniques to the most specialised tests like Tumor markers, therapeutic drug monitoring and clinical toxicology using Toxi-lab Screening by chromatographic & FPIA techniques.
The lab carries out stringent quality control for all tests, which are, performed either by the analysers, which is done at 3 levels twice a day or are done manually. The lab also participates in external Quality Control programmes from WHO and College of American Pathologists in USA.
The P. D. Hinduja Hospital Blood Bank is one among the few blood banks in the private sector which has the approval of the Food and Drug Administration (FDA) for preparing and issuing Blood Components to patients. Providing "safe blood" to the patients is the main aim of our blood bank. To ensure this, blood is collected only from healthy friends and relatives of patients who volunteer to do so.
A strict screening is carried out to exclude professional donors from donating blood units thus uaranteeing
safety and quality. Further, all units of blood are screened for HIV antibodies HBsAg, HCV antibody, VDRL, etc. 90% of the blood collected is separated into various components such as packed RBC, platelets, cryoprecipitate and plasma.
Thus the patient is given only the component he/she is deficient in, thereby avoiding the side effects of whole blood transfusion. All types of blood components are available round-the-clock in the blood bank. Availability of machines such as Cell Separator allows us to harvest specific blood components in pure form & in large quantities from a 'single' donor.
The quality of blood components is closely monitored to ensure efficacy & safety. We carry out a large number of 'apheresis' procedures using the latest Cobe Spectra cell separator, in adults as well as in children. Apart from platelet apheresis which is done routinely, Therapeutic plasma exchange is also carried out in a variety of disorders like Hemolytic-Uraemic- Syndrome, Myaesthenia Gravis and Gullain-Barre Syndrome. We are also amongst one of the few centres carrying out Peripheral blood stem cell (PBSC) collection and cryopreservation.
Hematology offers an exhaustive menu of investigations. Routine blood counts are done by top of the line equipment such as LH 750 from Beckman Coulter and Sysmex XE-2100 Blood Cell Analysers which give multiple parameters for each blood cell in every sample within a minute. The equipments generate interpretative data, along with flags or signals for abnormalities of red cells, white cells and platelets. All such 'flagged' samples are reviewed by the hematologist and a descriptive report with advice is issued to the patient.
A large variety of tests for characterizing complex bleeding and thrombotic disorders are available. These include coagulation factor assays, tests for thrombophilia and platelet function tests. Reliable monitoring of oral anticoagulation is made possible with the help of high quality reagents.
Other specialized investigations being carried out include study of abnormal hemoglobins by high performance liquid chromatography (HPLC), hemolytic anemia studies, leukemia cytochemistry etc.
We also have a FacCanto II Flow Cytometer which is capable of performing a 6- colour analysis simultaneously. This is a powerful tool for diagnosis, monitoring, staging & prognosis of a large number of hematological malignancies (acute leukemias and lymphomas) & immunological disorders (immune deficiency syndromes such as HIV).
Molecular test for diagnosing thrombotic disorders and some myeloproliferative diseases are also available to facilitate diagnosis of these less commonly occurring conditions.
Thrombocytopenia, a frequently occurring complication of heparin therapy is known as heparin induced thrombocytopenia (HIT). 1 – 5% of patients receiving heparin develop heparin induced thrombocytopenia. This is seen 5 – 15 days after starting heparin therapy if the patient is not previously exposed to heparin.
Clinical symptoms associated with HIT syndrome primarily include low platelet count and an increased risk of venous and/or arterial thromboembolism and the development of antibodies against a complex of heparin and Platelet Factor 4 (PF4). The HIT antibody test reveals detectable antibodies against the complex.
The Hematology Department has introduced a new test, the HIT Antibody Test. This is a Particle Gel Immuno Assay which reveals detectable antibodies against the heparin and Platelet Factor 4 (PF4) complex.
Sample Requirement: Blood (3-4 ml) sample should be drawn into Plain tube (no anticoagulant) as serum is required for the test. Patient need not be fasting.
Report Availability: The test will be performed on routine basis. Report of samples received by 1pm, available same day after 6 pm, else next day.
Histopathology offers services for processing of surgical specimens. In addition, 'frozen section' service is provided for urgent diagnosis, enzyme/muscle studies, fat stains, etc. Specialised study of kidney biopsies in chronic renal disorders & transplants by Immunofluorescence and Special Stains is possible due to the availability of the fluorescence microscope.
For more objective diagnosis of tumours, the Immunoperoxidase study utilising Monoclonal Antibodies and Immunohistochemical technique is available which makes diagnosis of undifferentiated and poorly differentiated tumours easier in more than 50% cases. This is also useful for hormone receptor analysis of tumors and oncogene studies.
The section also performs immunoperoxidase studies for dystrophin in frozen muscle tissue of muscular disorders. All surgical specimens are processed by standard technique and reported with grading and staging of tumours according to internationally used classifications. The cytopathology laboratory handles both gynaecological and non-gynaecological specimens sent for cytology.
About half of these are gynaecological 'PAP smears' for early detection of cervix cancer. The section has a standard cytospin for preparing concentrated smears of body fluids with low cell count or of small volume specimens like CSF, Urine etc. Fine needle Aspiration Cytology (FNAC) is also carried out for all palpable swellings in the body as the first line of investigative workup before a surgical biopsy.
Pneumocystis carinii detection in respiratory secretions is carried out by the immunofluorescent technique.
In addition to routine tests like Widal, VDRL, RA, CRP, etc., infection and autoimmune disease serology is performed. Serological investigations for "difficult to grow" pathogens as Mycoplasma, Leptospira, Pneumocystis, Cryptosporidium, Cryptococcus neoformans, Helicobacter pylori, Dengue, etc. are performed by Enzyme Immuno Assays, Immuno-fluorescence & Immunochromatographic techniques.
Polymerase Chain Reaction (PCR) for TB, HBVDNA, etc., is also carried out for rapid diagnosis of infection. ELISA Readers facilitate accurate results to ensure good sensitivity & specificity.
The Microbiology Laboratory is well equipped with sophisticated systems such as Bactec 9050 for Blood Culture (the percentage of recovery can reach upto 30%) and ATB (Automated Testing in Bacteriology).
Antimicrobial susceptibility testing is performed both by disc diffusion & at MIC breakpoints. NCCLS (National Committee for Clinical Laboratory Standards, USA) are followed for antimicrobial testing.
Rapid results for ICU and urgent cases are available within 6-8 hrs instead of 24 hrs by conventional methods. TB cultures are done by TB Bactec which is a rapid method (10 days to 3 weeks instead of 6 weeks by the conventional methods).
Anti TB drug susceptibility is also faster (12 days instead of 3 - 6 weeks We have got molecular based technologies as Transcription Mediated Amplification (TMA), and TB PCR also available with us (Result within 48 hrs).
In addition phage assay for Mycobacterium tuberculosis gives results within 48 hrs. Apart from routine isolation & identification of all clinical samples, aerobic, anaerobic, fungal & mycobacterial cultures are also done.
Smears for Acid Fast Bacilli are done on all samples routinely. Antimicrobial resistance data are kept for 10 yrs. Routinely microbiology surveillance of Operation Theatres & high-risk areas is carried out.
Definite policies for use of antibiotics, disinfectants & waste disposal are laid down by the Infection Control Committee and followed regularly. Protocols for all procedures are put up at all Nursing stations.
Needle-stick / post exposure prophylaxis (PEP) are followed for all Healthcare workers (HCWs) upto 6 months. Regular surveillance of all nosocomial infections and spot surveillance records are maintained.
(Cost Rs.10,500 each)
The RIA section of laboratory Medicine offers an array of investigations making use of unique technologies of Radioimmunoassay, Enzyme immunoassay and Chemiluminescence based immuno as says.
This section at P. D. Hinduja Hospital was the first in India to acquire “Immulite” and “Immulite 2500” fully automated random access enzyme enhanced chemiluminescence based immunoassay systems. The department has many firsts to its credit for introducing a number of unique and exclusive tests for which samples were sent abroad. Besides, four clinically relevant and crucial tests have been developed exclusively by this section benefiting both clinicians and patients. In addition to routine diagnostics for thyroid, Reproductive and Adrenal hormones the department offers the following exclusive tests.
The most advanced testing for various neuro markers is now available with us alone. They include:
Inbalance between angiogenesis-related factors is closely related to the development of pre-eclampsia. Pre-eclampsia is a multi system disorder that complicates upto 10% of all pregnancies. It is associated with substantial and significant morbidity and mortality of the mother and the baby. The clinical manifestations of pre-eclampsia consist mainly of hypertension, proteinuria, life threatening hematologic, hepatic, neuro logical problems along with obstetric complications. With the advent of specific immunoassays, it is now possible to measure the circulating angiogenic and anti-angiogenic proteins responsible for patho biology of pre-eclampsia.
According to a multicentre study conducted by WHO, women at risk of developing pre-eclampsia should be monitored before 20 weeks, at 24-28 weeks and 31-35 weeks of gestation for better management of pre-eclampsia and eclampsia.
It is now documented that measuring the levels of Vascular Endothelial Growth Factor (VEGF), Placental Growth Factor (PIGF), Endothelins and Soluble Fms like Tyrosine Kinase (SFIT-1) can predict the disease appearance and / or confirm the diagnosis when hypertension complicates a pregnancy.
There is now compelling data that indicates measuring blood levels of sFlt-1, VEGF and PIGF can predict pre-eclampsia more than 5 weeks prior to clinical manifestation of the disorder.
Since early prediction of pregnancy related complications can help save lives, these predictive markers will prove to be very useful in countries like ours, where tertiary care is limited and also distant.
Worried about being allergic to drugs? We provide highly standardised protocol based CAP accredited testing for numerous drugs.These include:
Total package costs Rs.11,340 only
For further details, please contact RIA Dept, Tel - 24447948
For getting yourself tested at home itself, Please call 28112323 / 9322504941
For any queries call RIA - 24447948
Lab based blood allergy testing is on the march making great strides in the field of in-vitro diagnostics. We at P. D. Hinduja Hospital were the first to introduce in vitro allergy testing in 1998 for more than 200 allergens in a single blood sample. We adopted a systematic approach to confirm or exclude allergies that a patient might be suffering from. A careful history of the patient and a single blood sample are all that we require to monitor your patient for any allergies including multi allergen panels for inhalants, molds, trees, weeds, grasses, animals, drugs and foods and individual allergens to exactly detect the offending allergen. All this is done on a fully automated chemiluminescence based Immulite 2500 analyser and allergen specific IgE reports are given within 24 hours.
All the results are subjected to strict quality assurance by participating in allergy Proficiency testing of College of American Pathologists.
Due to simplicity and accuracy of the one prick blood based allergy tests, we have seen an increase of upto 50 % in the number of patients being referred to us in the last three years.
Housevisit numbers : 28112323/9322504941
Critical evaluation of our patient data shows that allergies to milk, nuts, wheat and molds are on the
rise besides house dust and Dermatophagoides. These studies highlight the value of evidence based decision making by the referring physician using allergen specific IgE blood test technology.
Please write to us at firstname.lastname@example.org or email@example.com Or call us on
022-24447948 We also provide for Home Collection of samples for testing.For details,call us on
Histopathology & Cytopathology
Microbiology & Serology
Blood Bank - HLA
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